Detection of Cytochrome b5 in Bovine Skeletal Muscle by Electrophoretic Immunoblotting Technique

NADH shuttle enzymes and cytochrome b5 reductase in human skeletal muscle: effect of strength training.

The main aim of this study was to investigate whether enzyme levels of the malate-aspartate and alpha-glycerophosphate shuttles and of cytochrome b5 reductase in human skeletal muscle are affected by strength training. Muscle biopsy samples from the deltoid muscle of the nondominant arm in untrained (n = 12) and strength-trained (n = 12) subjects were compared. The strength-trained muscles were...

Unusual dehydroxylation of antimicrobial amidoxime prodrugs by cytochrome b5 and NADH cytochrome b5 reductase.

Furamidine is an effective antimicrobial agent; however, oral potency of furamidine is poor. A prodrug of furamidine, 2,5-bis(4-amidinophenyl)furan-bis-O-methylamidoxime (DB289), has greatly improved oral potency. DB289 is transformed to furamidine via O-demethylation, and N-dehydroxylation reactions with four intermediate metabolites formed. The O-demethylation reactions have been shown to be ...

Serodiagnosis of contagious bovine pleuropneumonia by immunoblotting

Reagent production: selecting the strain, preparing the antigen and controlling quality The choice of the strain to use is an extremely critical point. The use of the B103 strain, the reference strain used by Regalla et al. in 1999 to develop the IBT method, is highly recommended and should be used to ensure consistent results among laboratories. The antigen is then prepared from a culture grow...

Unusual Dehydroxylation of Antimicrobial Amidoxime Prodrugs by Cytochrome b5 and NADH Cytochrome b5 Reductase a

Kinetic investigation of carbon nanotube deposition by DC electrophoretic technique

In this paper, kinetics of DC electrophoretic deposition EPD of carbon nanotubes CNTs is investigated. Carbon nanotubes suspended in pure ethanol with addition of magnesium nitrate was used as deposition media. The effect of main EPD parameters such as deposition time, applied voltage and the CNT concentration on deposit yield was investigated. The variation of current density vs. time and the ...